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Human TRC1.5 library (LMBP LIB30)
Library name: | Human TRC1.5 library |
Library type: | shRNA |
Library accession number: | LMBP LIB30 |
Organism: | Homo sapiens |
Available clones: | See Sigma Aldrich for the available mouse TRC1.5 clones |
Vector name: | pLKO.1-puro-shRNA |
Vector sequence file: | p7210.txt |
Vector circular map: | p7210.pdf |
Vector accession number: | LMBP 7210 |
Vector type: | lentiviral vector |
Cloning sites: | AgeI-EcoRI |
Library coverage: | 17567 |
Average insert size: | 57 bp |
Distributed as: | individual clones as active cultures or plasmid DNA |
Further information: | The host strain of the individual clones is Escherichia coli K12 DH5αT1R. The individual clones can be grown in selective medium (TB-8gly + 100 µg/ml ampicillin) and cultivated at 37°C. pLKO.1-puro-shRNA (LMBP 7210) can be considered as a representative for all TRCN plasmids available at BCCM/GeneCorner. Check https://www.sigmaaldrich.com/life-science/functional-genomics-and-rnai/shrna/individual-genes.html for the available mouse TRC1.5 clones or contact BCCM/GeneCorner. |
History of deposit: | The library clones were purchased from Sigma-Aldrich in the frame of the Hercules project "RNAi collection and associated facilities". Schnelldorf, Germany |
Library reference: | - [PMID: -] |
Related reference: | Stewart et al., RNA 9 (2003), 493-501 [PMID: 12649500] Moffat et al., Cell 124 (2006), 1283-1298 [PMID: 16564017] |
Restricted distribution: | - Restricted to project-related users - The buyer cannot sell or transfer this product, its components nor materials made using this product or its components to another research group. - Visit www.sigma.com/shRNA for more, up-to-date info on the limited use of this product - Acknowledge BCCM/GeneCorner (supplier) and the Hercules Foundation (funder) in any publication resulting from the use of a shRNA clone |
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